Sequence: LIRLWSHLIHIWFQNRRLKWKKK-NH2
| Experiment Id | EXP000670 |
|---|---|
| Paper | Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic |
| Peptide | EB1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | no |
| Peptide Concentration | CR 4/1 |
| Rna Concentration | 200 nM |
| Mixing Ratio | charge ratio 4/1 |
| Formulation Format | CPP/siRNA polyplex |
| Formulation Components | EB1 peptide + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | K562 (CML); A549; Caco-2 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / proliferation inhibition |
| Output Value | ~72% BCR-ABL1 mRNA reduction at 24 h; ~50% proliferation inhibition |
| Output Units | |
| Output Notes | Efficient uptake via endocytosis; siRNA largely retained in endosomes/lysosomes with limited escape compared to liposomes; functional knockdown demonstrated. |
| Toxicity Notes | Low toxicity across time points. |
| Curation Notes |