Cell-penetrating peptide-conjugated lipid nanoparticles for siRNA delivery (2014)
Sequence: RRRRRRGGRRRRG
| Experiment Id | EXP000680 |
|---|---|
| Paper | Cell-penetrating peptide-conjugated lipid nanoparticles for siRNA delivery |
| Peptide | RRRRRRGGRRRRG |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 6 mol% (surface CPP) |
| Rna Concentration | 24–60 pmol per well |
| Mixing Ratio | CPP-LNP formulation |
| Formulation Format | CPP-decorated lipid nanoparticle |
| Formulation Components | RRRRRRGGRRRRG (protamine core) |
| Size Nm | 435.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | B16F10-luc2; EGFP/HT1080 |
| Animal Model | |
| Administration Route | |
| Output Type | reporter gene knockdown |
| Output Value | ~70% luciferase knockdown; strong EGFP reduction |
| Output Units | |
| Output Notes | Cytoplasmic siRNA localization with minimal lysosomal colocalization; uptake via macropinocytosis and heparan sulfate-mediated endocytosis. |
| Toxicity Notes | No significant cytotoxicity observed. |
| Curation Notes |