Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides (2016)
Sequence: Ac-C(WR)4CG-NH2
| Experiment Id | EXP000686 |
|---|---|
| Paper | Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides |
| Peptide | WR4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 100 µM |
| Rna Concentration | 100–600 nM |
| Mixing Ratio | 100–1000:1 (peptide:siRNA) |
| Formulation Format | CPP/siRNA noncovalent complex |
| Formulation Components | WR4 cyclic CPP + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549; H441 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | >90% knockdown (WR4); ~85% (L-FKFE); ~20–30% (D-FKFE) |
| Output Units | |
| Output Notes | WR4 shows strongest cytosolic delivery and knockdown; D-peptide less effective due to reduced unpackaging. |
| Toxicity Notes | No significant toxicity observed. |
| Curation Notes |