Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides (2016)
Sequence: Ac-C(WR)4CG-NH2
| Experiment Id | EXP000687 |
|---|---|
| Paper | Functional Delivery of siRNA by Disulfide-Constrained Cyclic Amphipathic Peptides |
| Peptide | WR4 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 100 µM |
| Rna Concentration | 100 nM |
| Mixing Ratio | 1000:1 (peptide:siRNA) |
| Formulation Format | CPP/siRNA noncovalent complex |
| Formulation Components | WR4 cyclic CPP + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mouse (lung aspiration) |
| Administration Route | intratracheal aspiration |
| Output Type | in vivo gene knockdown |
| Output Value | Reduced TTF-1 protein levels in lung tissue |
| Output Units | |
| Output Notes | Both L- and D-peptides show knockdown; L-forms more effective; WR4 strongest. |
| Toxicity Notes | No observable lung toxicity reported. |
| Curation Notes |