Rationally designed cationic amphiphilic peptides for selective gene delivery to cancer cells (2022)
Sequence: CIIKKIIKKIIKK-NH2
| Experiment Id | EXP000688 |
|---|---|
| Paper | Rationally designed cationic amphiphilic peptides for selective gene delivery to cancer cells |
| Peptide | IK-13 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | varied (N/P 1–5) |
| Rna Concentration | 25–30 nM siRNA |
| Mixing Ratio | N/P 1–5 |
| Formulation Format | CPP/nucleic acid noncovalent complex |
| Formulation Components | IK-13 peptide + siRNA/ODN |
| Size Nm | 90.00 |
| Zeta Mv | 2.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HCT116 colorectal cancer; HDF fibroblasts |
| Animal Model | |
| Administration Route | |
| Output Type | uptake and gene silencing |
| Output Value | IK-13, IR-13, CI-15 show functional silencing; LK-13, LR-13 uptake only |
| Output Units | |
| Output Notes | Leucine-rich peptides fail to release siRNA; isoleucine-rich show cytosolic release |
| Toxicity Notes | Low toxicity; cancer-selective effects |
| Curation Notes |