Sequence: LWRLWRLLWRLWRLL
| Experiment Id | EXP000695 |
|---|---|
| Paper | WRAP-based nanoparticles for siRNA delivery: a SAR study and a comparison with lipid-based transfect |
| Peptide | W1-2L |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | R=20 (~200 nM) |
| Rna Concentration | 10–20 nM |
| Mixing Ratio | 20:1 (peptide:siRNA) |
| Formulation Format | CPP-based nanoparticle |
| Formulation Components | W1-2L WRAP peptide + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 glioblastoma (FLuc/NLuc) |
| Animal Model | |
| Administration Route | |
| Output Type | luciferase knockdown |
| Output Value | variable (0–90%) depending on sequence |
| Output Units | |
| Output Notes | SAR study; helix length and Leu doublets critical for activity |
| Toxicity Notes | Low short-term toxicity |
| Curation Notes |