Lactosome-Conjugated siRNA Nanoparticles for Photo-Enhanced Gene Silencing in Cancer Cells (2021)
Sequence: LLLLLLLLIRLWSHLRIIHIWFQNRRLKWK
| Experiment Id | EXP000728 |
|---|---|
| Paper | Lactosome-Conjugated siRNA Nanoparticles for Photo-Enhanced Gene Silencing in Cancer Cells |
| Peptide | L7EB1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 12.5 mol% relative to Lactosome polymer |
| Rna Concentration | 1 mol% relative to Lactosome polymer |
| Mixing Ratio | PLLA-SS-siRNA incorporated during micelle formation |
| Formulation Format | CPP-modified polymeric micelle (Lactosome) |
| Formulation Components | A3B-type Lactosome + L7EB1 + TPFPP photosensitizer + siABCG2 |
| Size Nm | 50.00 |
| Zeta Mv | 2.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | PANC-1; NCI-H226; CHO |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~75–85% ABCG2 mRNA reduction after photoirradiation |
| Output Units | |
| Output Notes | L7EB1 alone enhances uptake but does not yield knockdown; photochemical internalization via TPFPP required for cytosolic siRNA delivery |
| Toxicity Notes | Low cytotoxicity without light; controlled phototoxicity upon PDT |
| Curation Notes |