Sequence: K4K2KGRKKRRQRRRPPQC (DEN-TAT) + perfluorocarbon (PFC)
| Experiment Id | EXP000729 |
|---|---|
| Paper | Fluorinated Cell-Penetrating Peptide for Co-Delivering siHIF-1α and Sorafenib to Enhance In Vitro An |
| Peptide | DEN-TAT-PFC |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ≤10 μM (functional studies) |
| Rna Concentration | 100 nM (in vitro) |
| Mixing Ratio | vector/siRNA molar ratio 75–100 |
| Formulation Format | peptide-based nanocomplex (co-loaded with small molecule) |
| Formulation Components | DEN-TAT-PFC + siRNA + sorafenib |
| Size Nm | 186.00 |
| Zeta Mv | 28.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2; HeLa; A375; HUVEC (angiogenesis assay) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown and anti-tumor activity |
| Output Value | ~70% HIF-1α mRNA knockdown; strong VEGF suppression; reduced tube formation |
| Output Units | |
| Output Notes | Fluorination improves uptake, serum resistance, and endosomal escape vs non-fluorinated DEN-TAT |
| Toxicity Notes | Low cytotoxicity and hemolysis below 10 μM |
| Curation Notes |