Sequence: CRGDKGPDC
| Experiment Id | EXP000730 |
|---|---|
| Paper | Endosomolytic and Tumor-Penetrating Mesoporous Silica Nanoparticles for siRNA/miRNA Combination Canc |
| Peptide | iRGD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | Typically 200 nM siRNA + 200 nM miRNA (+ ICG 1–3 µg/mL); some assays 300–400 nM |
| Mixing Ratio | siRNA:miRNA=1:1 (w/w); MSN:RNAs=30:1 (w/w); lipids:MSN=2:1 (w/w); fixed ICG:RNA = 1 µg/mL : 200 nM (per RNA) |
| Formulation Format | iRGD-modified lipid-coated mesoporous silica nanoparticle (iMSN) with ICG photosensitizer |
| Formulation Components | NH2-MSN core (100–200 nm, 4 nm pores) adsorbing siRNA+miRNA; ICG loaded; lipid coat SPC/DSPE-mPEG/DSPE-PEG-DBCO (2:2:1 w/w/w); iRGD (azido) click-conjugated (DSPE-PEG-DBCO:iRGD 1:1 molar) |
| Size Nm | 100.00 |
| Zeta Mv | -19.00 |
| Model Scope | in_vitro |
| Model Type | in vitro (2D cell culture + 3D tumor spheroids) |
| Cell Lines Or Primary Cells | MDA-MB-231 (incl. Gal8-YFP), MDA-MB-231.Luc, B16F10.Luc; 3D MDA-MB-231 spheroids |
| Animal Model | |
| Administration Route | Incubation with nanoparticles; 808 nm laser irradiation (typically 30 s after ~4 h incubation) for endosomal escape |
| Output Type | in vitro gene knockdown + functional effects |
| Output Value | Plk1 and ZEB1 mRNA downregulated (qRT-PCR) with light; luciferase knockdown reported; cell killing/migration inhibition shown |
| Output Units | |
| Output Notes | Endosomal escape demonstrated by Lysotracker colocalization decrease and Gal8 recruitment upon light-triggered ROS from ICG; uptake/penetration shown by confocal + flow cytometry in 3D spheroids (highest FAM/ICG double-positive with iMSN). |
| Toxicity Notes | Dose-dependent cytotoxicity with light; no standalone severe toxicity reported in vitro beyond treatment effects |
| Curation Notes |