Virus-mimetic polymeric micelles for targeted siRNA delivery (2010)
Sequence: KACDCRGDCFCG
| Experiment Id | EXP000732 |
|---|---|
| Paper | Virus-mimetic polymeric micelles for targeted siRNA delivery |
| Peptide | RGD4C |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 100–200 nM (in vitro) |
| Mixing Ratio | siRNA:polymer 1:16 (w/w) |
| Formulation Format | Peptide-functionalized polymeric micelles |
| Formulation Components | PEO-b-P(CL-g-polyamine) micelles with spermine core; peptide on PEO shell |
| Size Nm | 85.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA435/LCC6 (P-gp overexpressing breast cancer cells) |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Gene knockdown and chemosensitization |
| Output Value | mdr1 mRNA and P-gp protein downregulation; increased DOX accumulation; increased cytotoxicity |
| Output Units | |
| Output Notes | Integrin αvβ3–mediated uptake; partial endosomal escape; ~48% mdr1 knockdown vs NON-micelles |
| Toxicity Notes | Carrier-only toxicity observed (~60% viability with scramble siRNA) |
| Curation Notes |