Sequence: CKRRMKWKK
| Experiment Id | EXP000734 |
|---|---|
| Paper | Thermal and magnetic dual-responsive liposomes with a cell-penetrating peptide–siRNA conjugate for e |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 100–250 nM (in vitro assays) |
| Mixing Ratio | Encapsulated as siRNA–CPP conjugate within liposomes |
| Formulation Format | Thermal and magnetic dual-responsive liposomes (siRNA-CPPs/TML) |
| Formulation Components | DPPC/MSPC/DSPE-PEG2000 liposomes + Fe3O4 magnetic nanoparticles + siRNA–CPP conjugate |
| Size Nm | 90.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 human breast cancer cells |
| Animal Model | |
| Administration Route | Cell incubation ± AC magnetic field (423 kHz) |
| Output Type | Gene silencing, uptake, apoptosis |
| Output Value | Significant c-Myc mRNA/protein knockdown and apoptosis under magnetic hyperthermia |
| Output Units | |
| Output Notes | CPP-mediated uptake confirmed by flow cytometry/confocal; LysoTracker colocalization loss shows endosomal escape |
| Toxicity Notes | No nonspecific toxicity reported in vitro |
| Curation Notes |