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EXP000735

Paper

Thermal and magnetic dual-responsive liposomes with a cell-penetrating peptide–siRNA conjugate for enhanced and targeted cancer therapy (2016)

Peptide

CPP (penetratin-derived)

Sequence: CKRRMKWKK

RNA

siRNA

All experiment fields

Experiment Id EXP000735
Paper Thermal and magnetic dual-responsive liposomes with a cell-penetrating peptide–siRNA conjugate for e
Peptide CPP (penetratin-derived)
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 1.2 mg/kg siRNA (i.v.)
Mixing Ratio siRNA–CPP conjugate encapsulated in liposomes
Formulation Format Thermal and magnetic dual-responsive liposomes (siRNA-CPPs/TML)
Formulation Components Thermosensitive lipids + Fe3O4 magnetic core + siRNA–CPP conjugate
Size Nm 90.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model MCF-7 xenograft nude mouse model
Administration Route Intravenous injection + magnetic targeting + AC magnetic field hyperthermia
Output Type Tumor growth inhibition and in vivo gene silencing
Output Value Significant tumor growth suppression, c-Myc mRNA/protein knockdown, improved tumor accumulation
Output Units
Output Notes Magnetic + thermal activation essential; free siRNA and CPP–siRNA alone ineffective in vivo
Toxicity Notes No significant body weight loss or acute toxicity reported
Curation Notes