Sequence: KKALLAHALHLLALLALHLAHALKKA
mRNA (also plasmid DNA in secondary experiments)
| Experiment Id | EXP000739 |
|---|---|
| Paper | LipoParticles: Lipid-Coated PLA Nanoparticles Enhanced In Vitro mRNA Transfection Compared to Liposo |
| Peptide | LAH4-L1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 90 ng mRNA per well (in vitro) |
| Mixing Ratio | mRNA:LAH4-L1 = 1:20 (w/w) typical; optimized down to 1:5 |
| Formulation Format | pLbL-formulated LipoParticles (lipid-coated PLA nanoparticles) |
| Formulation Components | PLA nanoparticle core + DSPC/DOTAP lipid coat + mRNA + LAH4-L1 peptide |
| Size Nm | 300.00 |
| Zeta Mv | 44.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; DC2.4 dendritic cells |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Reporter protein expression and transfection efficiency |
| Output Value | Robust luciferase and eGFP expression; LP > liposomes |
| Output Units | |
| Output Notes | Endosomal escape via histidine-rich LAH4-L1; pH-dependent proton sponge mechanism |
| Toxicity Notes | Significant toxicity in DC2.4 at high peptide ratios; mitigated by lowering peptide content |
| Curation Notes |