Sequence: LKKLLKR8LKKLLKS5AG
| Experiment Id | EXP000743 |
|---|---|
| Paper | Construction of histidine-containing hydrocarbon stapled cell penetrating peptides for in vitro and |
| Peptide | stDK |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50 nM |
| Mixing Ratio | siRNA:peptide 1:10 to 1:50 |
| Formulation Format | Non-covalent peptide–siRNA complexes |
| Formulation Components | Stapled CPP electrostatically complexed with siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Gene knockdown |
| Output Value | >90% knockdown (only LKH-stEK); ≤60% others |
| Output Units | |
| Output Notes | Endosomal escape enhanced only for His-containing variants |
| Toxicity Notes | |
| Curation Notes |