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EXP000747

Paper

Construction of histidine-containing hydrocarbon stapled cell penetrating peptides for in vitro and in vivo delivery of siRNAs (2018)

Peptide

LKH-stEK

Sequence: LKHLLHLRKRHLLKLSG

RNA

siRNA

All experiment fields

Experiment Id EXP000747
Paper Construction of histidine-containing hydrocarbon stapled cell penetrating peptides for in vitro and
Peptide LKH-stEK
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 10–100 nM (intradermal)
Mixing Ratio siRNA:peptide 1:50
Formulation Format Non-covalent peptide–siRNA complexes
Formulation Components LKH-stEK + asiCTGF
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Mouse skin wound healing model
Administration Route Intradermal injection
Output Type In vivo gene silencing
Output Value Significant CTGF knockdown; reduced collagen deposition
Output Units
Output Notes
Toxicity Notes
Curation Notes