Sequence: Stearyl-AGYLLGKINLKALAALAKKIL-NH2
siRNA; splice-correcting oligonucleotide; plasmid DNA
| Experiment Id | EXP000748 |
|---|---|
| Paper | Effects of cargo molecules on membrane perturbation caused by transportan10 based cell-penetrating p |
| Peptide | PF3 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM siRNA; MR10 SCO; CR3 pGL3 |
| Mixing Ratio | CPP/siRNA MR20; CPP/SCO MR10; CPP/pGL3 CR3 |
| Formulation Format | Non-covalent peptide–nucleic acid complexes |
| Formulation Components | PF3 peptide + nucleic acid cargo |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; HeLa pLuc705 |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Uptake and functional nucleic acid delivery |
| Output Value | NF51: ~90% siRNA knockdown; NF1 ~60%; PF3 ~30% |
| Output Units | |
| Output Notes | Extensive biophysical and cellular uptake analysis; endosomal escape correlates with membrane leakage |
| Toxicity Notes | No major toxicity at working concentrations |
| Curation Notes |