Sequence: PF6 = Stearyl-AGYLLGKNLKALAALAKKIL-NH2 + 3×QN; R8 = stearyl-octaarginine
siRNA (+ plasmid DNA for stabilization)
| Experiment Id | EXP000753 |
|---|---|
| Paper | Development of a Novel Nanoparticle by Dual Modification With the Pluripotential Cell-Penetrating Pe |
| Peptide | PF6/R8-NP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P 3.0; siRNA:pDNA 1:2 |
| Formulation Format | Lipid-coated peptide polyplex nanoparticles |
| Formulation Components | PF6 or R8 polyplex core + DOPE/CHEMS lipid coat + PF6 or R8 surface |
| Size Nm | 250.00 |
| Zeta Mv | 15.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | B16-F1-luc |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Luciferase knockdown |
| Output Value | PF6/PF6-NP ~95% knockdown; R8/PF6-NP ~80%; PF6/R8-NP ~40%; R8/R8-NP ~0% |
| Output Units | |
| Output Notes | PF6 on both core and surface gives strongest RNAi via uptake, endosomal escape, and decondensation |
| Toxicity Notes | No significant toxicity; slight protein reduction for PF6/PF6-NP |
| Curation Notes |