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EXP000755

Paper

Development of a Novel Nanoparticle by Dual Modification With the Pluripotential Cell-Penetrating Peptide PepFect6 for Cellular Uptake, Endosomal Escape, and Decondensation of an siRNA Core Complex (2013)

Peptide

PF6 polyplex

Sequence: PF6 = Stearyl-AGYLLGKNLKALAALAKKIL-NH2 + 3×QN

RNA

siRNA (+ plasmid DNA)

All experiment fields

Experiment Id EXP000755
Paper Development of a Novel Nanoparticle by Dual Modification With the Pluripotential Cell-Penetrating Pe
Peptide PF6 polyplex
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio N/P 3.0; siRNA:pDNA 1:2
Formulation Format Peptide–siRNA polyplex (intermediate)
Formulation Components PF6 + siRNA + plasmid DNA
Size Nm 110.00
Zeta Mv 4.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells B16-F1-luc
Animal Model
Administration Route Not applied as standalone delivery system
Output Type Polyplex characterization
Output Value Loose condensation; positive surface charge
Output Units
Output Notes Characterized by DLS, AFM, hemagglutination; no functional RNAi tested alone
Toxicity Notes
Curation Notes