Acylation of octaarginine: Implication to the use of intracellular delivery vectors (2011)
Sequence: RRRRRRRR
| Experiment Id | EXP000773 |
|---|---|
| Paper | Acylation of octaarginine: Implication to the use of intracellular delivery vectors |
| Peptide | R8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 10 μM |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–small molecule conjugate (Alexa488) |
| Formulation Components | R8-Alexa488 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | Cell incubation |
| Output Type | Cellular uptake and intracellular distribution |
| Output Value | C6R8 shows highest uptake and diffuse cytosolic distribution |
| Output Units | |
| Output Notes | C6R8 and C10R8 show cytosolic diffusion; R8 and C4R8 remain endosomal |
| Toxicity Notes | C10R8 shows membrane perturbation; C6R8 non-toxic |
| Curation Notes |