An intracellular delivery method for siRNA by an arginine-rich peptide (2007)
SR9 (synthetic nona-arginine; R9)
Sequence: RRRRRRRRR
| Experiment Id | EXP000780 |
|---|---|
| Paper | An intracellular delivery method for siRNA by an arginine-rich peptide |
| Peptide | SR9 (synthetic nona-arginine; R9) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 200 nM |
| Mixing Ratio | N/P = 12 |
| Formulation Format | noncovalent peptide/siRNA complexes |
| Formulation Components | SR9 peptide + EGFP siRNA (mixed in PBS; 10 min cell treatment) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | GC-EGFP (human gastric carcinoma cells stably expressing EGFP; CRL-1739 derived) |
| Animal Model | |
| Administration Route | |
| Output Type | EGFP fluorescence knockdown |
| Output Value | 43% decrease vs control |
| Output Units | |
| Output Notes | GC-EGFP cells treated 10 min with SR9+siRNA complexes, then cultured 48 h; quantified by confocal microscopy (Fig. 5). |
| Toxicity Notes | SR9 reported almost non-toxic in GC-EGFP (3.5–10.5 µM; trypan blue) and A549 cells (15 µg/mL; MTT) (Fig. 7). |
| Curation Notes |