Sequence: CGRRMKWKK-[photo]-EEEERRRR
| Experiment Id | EXP000782 |
|---|---|
| Paper | Polymer nanoparticles modified with photo- and pH-dual-responsive polypeptides for enhanced and targ |
| Peptide | PPP (non-activated) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | PLGA nanoparticles (w/o/w double emulsion) PEGylated with DSPE-PEG2000; surface ligand DSPE-PEG2000-peptide; siRNA encapsulated as siRNA–spermidine complex |
| Formulation Components | PLGA (50/50); DSPE-PEG2000; PVA; siRNA–spermidine complex |
| Size Nm | 116.27 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 (human breast adenocarcinoma) |
| Animal Model | |
| Administration Route | |
| Output Type | EGFR gene knockdown (qRT-PCR / western blot) |
| Output Value | |
| Output Units | |
| Output Notes | Same PPP-NPs formulation but without NIR activation (dark). Uptake was low and similar to non-modified NPs at both pH 7.4 and 6.0. EGFR-siRNA delivered by PPP-NPs without NIR showed weaker silencing than activated PPP-NPs. Encapsulation efficiency ~87%. |
| Toxicity Notes | |
| Curation Notes |