Sequence: CGRRMKWKK
| Experiment Id | EXP000783 |
|---|---|
| Paper | Polymer nanoparticles modified with photo- and pH-dual-responsive polypeptides for enhanced and targ |
| Peptide | CPP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | PLGA nanoparticles (w/o/w double emulsion) PEGylated with DSPE-PEG2000; surface ligand DSPE-PEG2000-peptide; siRNA encapsulated as siRNA–spermidine complex |
| Formulation Components | PLGA (50/50); DSPE-PEG2000; PVA; siRNA–spermidine complex |
| Size Nm | 113.68 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 (human breast adenocarcinoma) |
| Animal Model | |
| Administration Route | |
| Output Type | EGFR gene knockdown (qRT-PCR / western blot) |
| Output Value | |
| Output Units | |
| Output Notes | CPP-NPs (10 mol% DSPE-PEG2000-CPP) showed high uptake (flow cytometry/confocal) and strong EGFR knockdown and apoptosis in MCF-7 cells. Encapsulation efficiency ~87%. |
| Toxicity Notes | |
| Curation Notes |