Sequence: RLLRLLLRLWRRLLRLLR
| Experiment Id | EXP000789 |
|---|---|
| Paper | Modification of a Designed Amphipathic Cell Penetrating Peptide and Its Effect on Solubility, Second |
| Peptide | C6 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50 nM |
| Mixing Ratio | 15:1 (peptide:siRNA molar ratio) |
| Formulation Format | noncovalent peptide–siRNA complex |
| Formulation Components | peptide + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1 |
| Animal Model | |
| Administration Route | |
| Output Type | uptake (flow cytometry Cy3) |
| Output Value | |
| Output Units | |
| Output Notes | Flow cytometry uptake after trypsin + heparin wash to remove surface-bound complexes; C6M1 showed ~90% higher uptake than C6 at same molar ratio. |
| Toxicity Notes | CCK-8 viability assay (24 h); reported IC50: C6 ≈12 µM; C6M1 ≈22 µM. Note: methods/figure indicate CHO-K1; text mentions A549. |
| Curation Notes |