The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA (2013)
Sequence: RKKRRQRRR
| Experiment Id | EXP000795 |
|---|---|
| Paper | The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | High |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2 µM (calculated from 50 nM siRNA × 40) |
| Rna Concentration | 50 nM |
| Mixing Ratio | 40:1 (CPP:siRNA) |
| Formulation Format | Noncovalent electrostatic CPP/siRNA complex |
| Formulation Components | TAT + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | Gene knockdown (GAPDH mRNA) |
| Output Value | 46% decrease at 48 h (35% at 24 h) |
| Output Units | |
| Output Notes | Knockdown quantified by qPCR; protein knockdown also observed by western blot. siRNA uptake measured by flow cytometry with 5-FAM siRNA. |
| Toxicity Notes | No significant decrease in HeLa cell viability or proliferation after 48 h incubation with bLFcin6, CPP5, CPP6, or TAT up to 80 µM (methylene blue and MTT assays). |
| Curation Notes |