The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA (2013)
Sequence: PFVYLI
| Experiment Id | EXP000796 |
|---|---|
| Paper | The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA |
| Peptide | CPP6 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | High |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50 nM |
| Mixing Ratio | Tested 1:1–80:1 (CPP:siRNA); no complex formed |
| Formulation Format | Attempted noncovalent electrostatic CPP/siRNA complex |
| Formulation Components | CPP6 + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | siRNA delivery / uptake |
| Output Value | No siRNA delivery detected |
| Output Units | |
| Output Notes | CPP6 did not form electrostatic complexes with siRNA at tested molar ratios and did not deliver siRNA into HeLa cells. |
| Toxicity Notes | No significant decrease in HeLa cell viability or proliferation after 48 h incubation with bLFcin6, CPP5, CPP6, or TAT up to 80 µM (methylene blue and MTT assays). |
| Curation Notes |