Photolabile-caged peptide-conjugated liposomes for siRNA delivery (2015)
Sequence: CGRRMKPGWKPGKPG
| Experiment Id | EXP000808 |
|---|---|
| Paper | Photolabile-caged peptide-conjugated liposomes for siRNA delivery |
| Peptide | PCP (NIR-uncaged) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 1.2 mg/kg siRNA |
| Mixing Ratio | |
| Formulation Format | cationic liposomes (peptide-PEG-DSPE surface modification) |
| Formulation Components | SPC/Chol/DC-Chol/DSPE-mPEG2000 liposomes; post-inserted DSPE-PEG2000-peptide at 3 mol% of total lipid; NIR irradiation at tumor site (740 nm, 30 min) to uncage PCP |
| Size Nm | 124.81 |
| Zeta Mv | 14.86 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | MCF-7 xenograft in female BALB/c nude mice |
| Administration Route | intravenous (tail vein); NIR irradiation at tumor site 4 h post-dose for NIR groups |
| Output Type | tumor doubling time (days) |
| Output Value | 4.33 |
| Output Units | |
| Output Notes | NC-siRNA control shows no tumor growth delay and no EGFR knockdown; confirms sequence-specific therapeutic effect for EGFR-siRNA. |
| Toxicity Notes | No significant body weight change reported. |
| Curation Notes |