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EXP000810

Paper

Targeting IgE polyadenylation signal with antisense oligonucleotides decreases IgE secretion and plasma cell viability (2022)

Peptide

Pip6a

Sequence: Ac-RXRRBRRXRYQFLIRXRBRXRB-acid (B=β-alanine; X=aminohexanoic acid)

RNA

Morpholino ASO (Vivo-morpholino / PMO)

All experiment fields

Experiment Id EXP000810
Paper Targeting IgE polyadenylation signal with antisense oligonucleotides decreases IgE secretion and pla
Peptide Pip6a
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 12.5 mg/kg (IV tail vein, single dose; serum measured 24 h later)
Mixing Ratio
Formulation Format CPP-conjugated ASO
Formulation Components Pip6a peptide conjugated to 3′ end of vivo-morpholino IgE-PAS ASO (Pip6a-ASO)
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model InEps/CreERT2+ humanized IgE mouse model; tamoxifen-induced IgE expression
Administration Route IV (tail vein)
Output Type Serum IgE
Output Value
Output Units
Output Notes Systemic Pip6a-ASO significantly decreased serum humanized IgE vs control ASO 24 h post-injection (Fig 5C). Exact numeric values not provided in text.
Toxicity Notes
Curation Notes