N-terminal modification of an LAH4-derived peptide increases mRNA delivery in the presence of serum (2024)
Sequence: KKALLHAALAHLLALAHHLLALLKKA
| Experiment Id | EXP000828 |
|---|---|
| Paper | N-terminal modification of an LAH4-derived peptide increases mRNA delivery in the presence of serum |
| Peptide | LAH4-A4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Screen: 2.5–12 µg peptide per 0.6 µg mRNA (U87); subsets used 5 µg peptide per 0.5 µg mRNA (HCT116). |
| Formulation Format | peptide/mRNA complexes (polyplex) |
| Formulation Components | Buffers used for complexation included 10 mM acetate pH5, 150 mM NaCl, or 10 mM phosphate buffer pH6.25 (also PBS, HBG in optimization). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 |
| Animal Model | |
| Administration Route | |
| Output Type | Functional reporter expression (luciferase and/or GFP) |
| Output Value | |
| Output Units | |
| Output Notes | In vitro mRNA transfection measured by luciferase reporter expression (U87 screen; selected peptides also tested in HCT116). Values reported in figures (not tabulated). |
| Toxicity Notes | |
| Curation Notes |