Sequence: RRRRRRRRR
plasmid DNA (pDNA; miRNA expression)
| Experiment Id | EXP000842 |
|---|---|
| Paper | MicroRNA delivery by arginine-rich cell-penetrating peptides: An investigation on expression and the |
| Peptide | R9 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 2.874 µg peptide : 1 µg DNA (w/w); complex in 150 µL then diluted to 300 µL |
| Formulation Format | CPP/pDNA complex (polyplex) |
| Formulation Components | R9 peptide + pLenti-III-miR-124-GFP plasmid |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh7 (human hepatocellular carcinoma) |
| Animal Model | |
| Administration Route | |
| Output Type | miR-124 expression (RT-qPCR fold-change vs empty vector) |
| Output Value | 2.5 |
| Output Units | |
| Output Notes | 48 h post-transfection; GFP reporter expression by fluorescence microscopy; miR-124 fold-change P=0.008. |
| Toxicity Notes | Low hemolysis vs Triton X-100; no significant difference vs PBS at tested concentrations. |
| Curation Notes |