Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure–activity studies (2008)
Sequence: (R-Ahx-R)4AhxB
| Experiment Id | EXP000873 |
|---|---|
| Paper | Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure–activity studies |
| Peptide | compound 5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | High |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1 µM (conjugate) |
| Rna Concentration | 1 µM (conjugate) |
| Mixing Ratio | |
| Formulation Format | covalent peptide–PMO conjugate |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 cells |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction (luciferase assay, RLU/µg protein) |
| Output Value | |
| Output Units | |
| Output Notes | Most active splice correction in spacing series at 1 µM; see Fig.1C. Uptake quantified by FACS; see Fig.4. Endosome-mimicking liposome leakage at pH 5.5 shown; see Fig.8. |
| Toxicity Notes | |
| Curation Notes |