Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure–activity studies (2008)
Sequence: R8
| Experiment Id | EXP000881 |
|---|---|
| Paper | Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure–activity studies |
| Peptide | R8 (Arg8) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | High |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 5 µM (liposome leakage assay) |
| Rna Concentration | 5 µM (liposome leakage assay) |
| Mixing Ratio | |
| Formulation Format | covalent peptide–PMO conjugate |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 cells |
| Animal Model | |
| Administration Route | |
| Output Type | endosome-mimicking liposome leakage (ANTS/DPX; late endosome lipid mix) at pH 5.5 vs 7.4 |
| Output Value | |
| Output Units | |
| Output Notes | Used as comparator in liposome leakage assay; see Fig.8. No splice-correction data shown for R8 in this paper. |
| Toxicity Notes | |
| Curation Notes |