Sequence: RQIKIWFQNRRMKWKK
| Experiment Id | EXP000898 |
|---|---|
| Paper | Structural Requirements for Cellular Uptake and Antisense Activity of Peptide Nucleic Acids Conjugat |
| Peptide | Penetratin |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 1 µM (conjugate in OptiMEM, 4 h incubation unless noted) |
| Rna Concentration | 1 µM (conjugate; covalent so same as peptide) |
| Mixing Ratio | covalent (1:1) |
| Formulation Format | covalent PNA–peptide conjugate |
| Formulation Components | Stable conjugate: PNA-Pen |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc 705 (luciferase/IVS2-705 splice-correction reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase splice-correction reporter |
| Output Value | |
| Output Units | |
| Output Notes | Confocal microscopy (FAM-PNA-Pen): punctate cytosolic fluorescence without agents (endosomal sequestration); chloroquine increased nuclear uptake. Splice-correction activity remained low without chloroquine; only slight enhancement with chloroquine. |
| Toxicity Notes | MAP-derived (KLA/RLA/ELA) conjugates showed cytotoxicity at higher concentrations (reported ~20% viability at 4 µM by MTT). |
| Curation Notes |