Cell-penetrating peptides for siRNA delivery to glioblastomas (2018)
Sequence: Stearyl-LLOOLAAAALOOLLTFFYGGSRGKRNNFKTEEY-NH2
| Experiment Id | EXP000907 |
|---|---|
| Paper | Cell-penetrating peptides for siRNA delivery to glioblastomas |
| Peptide | PF32 (PepFect 32) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 25 nM |
| Mixing Ratio | MR 20 (and others) tested |
| Formulation Format | peptide/siRNA non-covalent nanocomplex |
| Formulation Components | ANG targeting moiety is part of PF32 sequence (covalent) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 MG-luc2 (human glioblastoma); HeLa-luc used as non-glioma control in supplemental experiments |
| Animal Model | |
| Administration Route | |
| Output Type | luciferase knockdown |
| Output Value | No knockdown reported |
| Output Units | |
| Output Notes | PF32 was not able to downregulate luciferase at 25 nM siRNA in U87 MG-luc2 (text; Fig.4) and did not improve PF14 at 10 nM (text; Fig.6). |
| Toxicity Notes | No significant toxicity reported in U87 MG-luc2 at 25 nM siRNA (WST-1, 24 h) across tested MRs for all peptide:siRNA complexes. |
| Curation Notes |