Cell-penetrating peptides for siRNA delivery to glioblastomas (2018)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLLC-(S-S)-CTFFYGGSRGKRNNFKTEEY-NH2
| Experiment Id | EXP000908 |
|---|---|
| Paper | Cell-penetrating peptides for siRNA delivery to glioblastomas |
| Peptide | PF38 (PepFect 38) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 10 nM |
| Mixing Ratio | MR 20 tested |
| Formulation Format | peptide/siRNA non-covalent nanocomplex |
| Formulation Components | ANG targeting moiety disulfide-linked; reducible (S-S) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 MG-luc2 (human glioblastoma); HeLa-luc used as non-glioma control in supplemental experiments |
| Animal Model | |
| Administration Route | |
| Output Type | luciferase knockdown |
| Output Value | No improvement vs PF14 reported |
| Output Units | |
| Output Notes | PF38 did not improve gene silencing compared to PF14 at low siRNA (text; Fig.6); quantitative knockdown not explicitly stated. |
| Toxicity Notes | No significant toxicity reported in U87 MG-luc2 at 25 nM siRNA (WST-1, 24 h) across tested MRs for all peptide:siRNA complexes. |
| Curation Notes |