D-Tat57-49 (retro-inverso Tat)
Sequence: rrrqrrkkr
| Experiment Id | EXP000910 |
|---|---|
| Paper | Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellul |
| Peptide | D-Tat57-49 (retro-inverso Tat) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10 µmol/kg (i.v. dose) |
| Rna Concentration | 10 µmol/kg (same as conjugate) |
| Mixing Ratio | |
| Formulation Format | covalent CPP–PNA–Gd(DOTA)–FITC conjugate |
| Formulation Components | CPP (D-Tat57-49) + AEEA spacer + PNA (12mer) + Lys(FITC) + Gd(DOTA) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mice |
| Administration Route | intravenous (tail vein) |
| Output Type | biodistribution |
| Output Value | Fluorescence detected in liver and bladder (high), kidney/spleen (moderate) at 20 min |
| Output Units | |
| Output Notes | Animals sacrificed 20 min post i.v. injection; tissue slices imaged for FITC fluorescence; no BBB penetration observed (brain negative). |
| Toxicity Notes | No conjugate-related toxicity observed (reported). |
| Curation Notes |