L5a (LFcin-derived penta-peptide)
Sequence: RRWQW
| Experiment Id | EXP000912 |
|---|---|
| Paper | Identification of a Short Cell-Penetrating Peptide from Bovine Lactoferricin for Intracellular Deliv |
| Peptide | L5a (LFcin-derived penta-peptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 16.0 µg (with 1.6 µg DNA; fluorescence assay); 40 µg (with 4 µg DNA; qPCR assay) |
| Rna Concentration | 1.6 µg (fluorescence assay) / 4 µg (qPCR assay) |
| Mixing Ratio | N/P = 12 (NH3+/PO4−) |
| Formulation Format | noncovalent CPP/DNA complexes |
| Formulation Components | L5a (LFcin-derived penta-peptide) peptide + plasmid DNA (reporter); complexes formed at N/P=12; some experiments included 113 mM CaCl2 |
| Size Nm | 199.20 |
| Zeta Mv | 4.80 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Human A549 lung carcinoma cells (ATCC CCL-185) |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro reporter expression (EGFP mRNA/protein) |
| Output Value | EGFP expression detected (qPCR and fluorescence microscopy); CaCl2 increased expression intensity without changing transfection efficiency |
| Output Units | |
| Output Notes | CPP uptake/internalization assessed by flow cytometry and fluorescence microscopy using FITC-labeled peptides; DNA complexation validated by gel retardation; reporter expression assessed 48 h post-treatment. Calcium chloride (113 mM) increased expression intensity but did not change transfection efficiency in reported assays. |
| Toxicity Notes | MTT: CPPs and CPP/DNA complexes showed ~100% viability; Lipofectamine/DNA showed mild cytotoxicity. |
| Curation Notes |