A convergent uptake route for peptide- and polymer-based nucleotide delivery systems (2015)
Sequence: Stearyl-AGYLLG(Ka)INLKALAALAKKIL-NH2
| Experiment Id | EXP000916 |
|---|---|
| Paper | A convergent uptake route for peptide- and polymer-based nucleotide delivery systems |
| Peptide | PepFect 6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported (complexed at optimal charge ratio; in vitro, 0.5–1 µg plasmid per well depending on assay) |
| Rna Concentration | 0.5–1 µg pGL3 (per well; 24-well plate) |
| Mixing Ratio | charge ratio (CR) optimized; used for assays (reported as CR in methods/figures) |
| Formulation Format | noncovalent CPP:pDNA complexes |
| Formulation Components | PF6 peptide + pGL3 luciferase plasmid DNA; uptake inhibited by SCARA inhibitors (dextran sulfate, poly I, fucoidin) vs controls |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro reporter expression (luciferase) |
| Output Value | Luciferase expression strongly inhibited/abolished by SCARA inhibitors (Dex/Poly I/Fuc) vs control inhibitors |
| Output Units | |
| Output Notes | Study shows CPP:pDNA complexes are taken up via class A scavenger receptors (SCARA), based on pharmacological inhibition; luciferase measured 24 h after transfection. |
| Toxicity Notes | |
| Curation Notes |