A convergent uptake route for peptide- and polymer-based nucleotide delivery systems (2015)
Sequence: Stearyl-RxRRxRRxRRxR-NH2 (x = 6-aminohexanoic acid)
| Experiment Id | EXP000919 |
|---|---|
| Paper | A convergent uptake route for peptide- and polymer-based nucleotide delivery systems |
| Peptide | s-RxR4 (stearylated (RxR)4) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported (complexed at CR5 for plasmid delivery per methods) |
| Rna Concentration | 1 µg pGL3 (per well; 24-well plate) or 0.5 µg pGL3Luc (per methods) |
| Mixing Ratio | CR5 (+/−) for plasmid delivery (reported for s-RxR4) |
| Formulation Format | noncovalent CPP:pDNA complexes |
| Formulation Components | s-RxR4 peptide + pGL3 luciferase plasmid DNA; reduced delivery after SCARA3/5 knockdown; increased delivery after SCARA3/5 overexpression |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro reporter expression (luciferase) |
| Output Value | Luciferase delivery reduced by SCARA3/5 siRNA; increased by SCARA3/5 overexpression |
| Output Units | |
| Output Notes | Structurally distinct CPP shows same SCARA-dependent uptake route for CPP:pDNA complexes. |
| Toxicity Notes | |
| Curation Notes |