A convergent uptake route for peptide- and polymer-based nucleotide delivery systems (2015)
Sequence: Stearyl-AGYLLG(Ka)INLKALAALAKKIL-NH2
| Experiment Id | EXP000920 |
|---|---|
| Paper | A convergent uptake route for peptide- and polymer-based nucleotide delivery systems |
| Peptide | PepFect 6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | MR 40:1 (peptide:siRNA); peptide amount not reported |
| Rna Concentration | final siRNA 50 nM |
| Mixing Ratio | MR 40:1 (peptide:siRNA) |
| Formulation Format | noncovalent CPP:siRNA nanocomplexes |
| Formulation Components | PF6 peptide + siRNAEGFP; uptake/functional effect blocked by SCARA3/5 knockdown |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U2OS-SAMP1-YFP cells (YFP reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro gene knockdown (YFP by FACS) |
| Output Value | YFP down-regulation in control cells; knockdown abolished when SCARA3/5 were silenced |
| Output Units | |
| Output Notes | SCARA3/5 siRNA pretreatment prevents functional delivery of siRNA by PF6 (FACS readout). |
| Toxicity Notes | |
| Curation Notes |