A convergent uptake route for peptide- and polymer-based nucleotide delivery systems (2015)
PF14-Fl (fluorescent PF14 analog)
Sequence: Stearyl-AGYLLG(Kc)LLOOLAAAALOOLL-NH2
| Experiment Id | EXP000922 |
|---|---|
| Paper | A convergent uptake route for peptide- and polymer-based nucleotide delivery systems |
| Peptide | PF14-Fl (fluorescent PF14 analog) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | not reported |
| Rna Concentration | not reported |
| Mixing Ratio | not reported (PF14-Fl:siRNA-alexa complexes used for microscopy) |
| Formulation Format | noncovalent CPP:siRNA complexes (fluorescent tracking) |
| Formulation Components | PF14-Fl peptide + siRNA-Alexa568; uptake blocked by SCARA inhibitors (Dextran sulfate, Poly I, Fucoidin) vs controls |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells |
| Animal Model | |
| Administration Route | |
| Output Type | uptake / intracellular localization (microscopy) |
| Output Value | Punctate intracellular fluorescence consistent with endosomal uptake; inhibitors blocked internalization |
| Output Units | |
| Output Notes | Microscopy shows widespread punctate fluorescence without inhibitors/control inhibitors; SCARA inhibitors block uptake of both PF14-Fl:siRNA and PF14-Fl alone. |
| Toxicity Notes | |
| Curation Notes |