VEGF siRNA Delivery by a Cancer-Specific Cell-Penetrating Peptide (2018)
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| Experiment Id | EXP000930 |
|---|---|
| Paper | VEGF siRNA Delivery by a Cancer-Specific Cell-Penetrating Peptide |
| Peptide | R9 (poly-arginine CPP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P=8 (1 h transfection) |
| Rna Concentration | not reported (readout at 48 h) |
| Mixing Ratio | N/P = 8 |
| Formulation Format | noncovalent CPP-siRNA complexes (electrostatic; N/P ratio) |
| Formulation Components | R9 peptide + siGFP |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | GFP-expressing HeLa and GFP-expressing NIH3T3 |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro knockdown (GFP fluorescence + qRT-PCR) |
| Output Value | HeLa: GFP fluorescence down 15.2% and GFP mRNA down 18.4%; NIH3T3: GFP fluorescence down 19.8% and GFP mRNA down 25.2% |
| Output Units | |
| Output Notes | CPP comparator; weaker knockdown in this study. |
| Toxicity Notes | No major toxicity emphasized. |
| Curation Notes |