Sequence: Stearyl-AGYLLGK(rYaOF-eN)LLOOLAAAALOOLL-NH2
| Experiment Id | EXP000937 |
|---|---|
| Paper | Intracellular delivery of therapeutic antisense oligonucleotides targeting mRNA coding mitochondrial |
| Peptide | mitFect3 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.3–1.0 µM (depends on MR; oligo 100 nM) |
| Rna Concentration | 100 nM |
| Mixing Ratio | MR 3:1, 5:1, 10:1 (peptide:SCO) |
| Formulation Format | non-covalent peptide/oligonucleotide nanocomplex |
| Formulation Components | Peptide + oligonucleotide in MilliQ water (10% final volume), incubated 1 h RT; added to cells in complete medium |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction (luciferase) |
| Output Value | Comparable to PF14 for splice correction (MR5/MR10) |
| Output Units | |
| Output Notes | Reported as efficient as PF14 for SCO splice correction. |
| Toxicity Notes | WST-1: >80% cell viability (tested MRs) |
| Curation Notes |