Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide (2018)
Sequence: KIPLPRFKLKCIFCKKRRKR
| Experiment Id | EXP000989 |
|---|---|
| Paper | Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide |
| Peptide | hPP10 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | pDNA 80 µl at 50 ng/µl used for complex formation (method); transfection conditions vary |
| Mixing Ratio | N/P=40–80 tested; improved with 5% DMSO pretreatment |
| Formulation Format | CPP/DNA complex (noncovalent) |
| Formulation Components | hPP10 + plasmid DNA (± 5% DMSO pretreatment) |
| Size Nm | 544.10 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Caski (also tested in B16, ECV304, HepG2 per methods) |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | |
| Output Units | |
| Output Notes | GFP expression observed after hPP10-mediated transfection; DMSO pretreatment improved efficiency at N/P 40 and 80. |
| Toxicity Notes | No significant cytotoxicity reported for hPP10/pDNA or TAT/pDNA at various N/P ratios in Caski and B16 after 24–48 h (MTT). |
| Curation Notes |