Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide (2018)
hPP10 (with hPP10-NLS-Cre protein)
Sequence: KIPLPRFKLKCIFCKKRRKR
| Experiment Id | EXP000992 |
|---|---|
| Paper | Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide |
| Peptide | hPP10 (with hPP10-NLS-Cre protein) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | hPP10/pDNA transfection then hPP10-NLS-Cre treatment (timing per figure legend) |
| Formulation Format | CPP/DNA complex + CPP-fusion protein transduction |
| Formulation Components | hPP10 + LGluc plasmid + hPP10-NLS-Cre fusion protein |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Caski |
| Animal Model | |
| Administration Route | |
| Output Type | reporter switch (GFP↓, luciferase↑) |
| Output Value | |
| Output Units | |
| Output Notes | After hPP10-NLS-Cre treatment, GFP-expressing cells decreased while luciferase activity increased, indicating Cre/loxp recombination after delivery. |
| Toxicity Notes | No significant cytotoxicity reported for hPP10/pDNA or TAT/pDNA at various N/P ratios in Caski and B16 after 24–48 h (MTT). |
| Curation Notes |