Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide (2018)
Sequence: H-YGRKKRRQRRR-NH2
| Experiment Id | EXP000993 |
|---|---|
| Paper | Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P=40–80 tested; minimal improvement with 5% DMSO per figure |
| Formulation Format | CPP/DNA complex (noncovalent) |
| Formulation Components | TAT + plasmid DNA (± 5% DMSO pretreatment) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Caski |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | |
| Output Units | |
| Output Notes | GFP expression observed for TAT/pDNA complexes; DMSO pretreatment showed little improvement vs hPP10 in the reported comparison. |
| Toxicity Notes | No significant cytotoxicity reported for hPP10/pDNA or TAT/pDNA at various N/P ratios in Caski and B16 after 24–48 h (MTT). |
| Curation Notes |