Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide (2018)
Sequence: H-YGRKKRRQRRR-NH4
| Experiment Id | EXP000995 |
|---|---|
| Paper | Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | with vs without 5% DMSO pretreatment compared |
| Formulation Format | CPP/DNA complex (noncovalent) |
| Formulation Components | TAT + plasmid DNA (± 5% DMSO pretreatment) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Caski, ECV304, B16 |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression (luciferase) |
| Output Value | |
| Output Units | |
| Output Notes | TAT-mediated Gluc transfection increased after 5% DMSO pretreatment. |
| Toxicity Notes | No significant cytotoxicity reported for hPP10/pDNA or TAT/pDNA at various N/P ratios in Caski and B16 after 24–48 h (MTT). |
| Curation Notes |