Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide (2018)
Sequence: KIPLPRFKLKCIFCKKRRKR
| Experiment Id | EXP000996 |
|---|---|
| Paper | Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide |
| Peptide | hPP10-E3 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | complexed in vitro (hPP10-E3/siGFP); 2 h incubation ± 5% DMSO pretreatment; readout at 24 h |
| Formulation Format | CPP-fusion protein/siRNA complex |
| Formulation Components | hPP10-E3 fusion protein + siRNA (± 5% DMSO pretreatment) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Stable GFP-expressing Caski |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | |
| Output Units | |
| Output Notes | hPP10-E3/siGFP treatment efficiently silenced GFP expression; DMSO pretreatment increased efficiency. |
| Toxicity Notes | |
| Curation Notes |