EXP001041

Paper

Sequence: LGAQSNF

Experiment Id	EXP001041
Paper	Hydrophobic CPP/HDO conjugates: a new frontier in oligonucleotide-warheaded PROTAC delivery
Peptide	P4
Delivery Success Class	no
In Vivo Flag	no
Uptake Confirmed	yes
Label Confidence	high
In Vitro Functional Effect	yes
Endosomal Escape Evidence	no
Peptide Concentration	1–10 µM tested; ERα degradation at 10 µM (24 h, no transfection)
Rna Concentration	1–10 µM (same as peptide-conjugate dose; treated as CPP/HDO-PROTAC)
Mixing Ratio	1:1 (LCL-HDO-F : P4-HDO-R; annealed duplex)
Formulation Format	annealed duplex; covalent P4 conjugation to antisense strand (P4-HDO-R, click chemistry)
Formulation Components	CPP/HDO-PROTAC = LCL-HDO-F (5′-LCL-GTCAGGTCACAGTGACCTGATCAAAGTTAA-3′) + P4-HDO-R (3′-CAGTCCAGTGTCACTGGACTAguuucaa uu-5′-P4)
Size Nm
Zeta Mv
Model Scope	in_vitro
Model Type	in vitro
Cell Lines Or Primary Cells	MCF-7 (human breast cancer cells; ERα-positive)
Animal Model
Administration Route
Output Type	ERα degradation; proliferation inhibition; uptake imaging
Output Value	ERα degradation at 10 µM after 24 h (no transfection); growth inhibition at 1–10 µM after 72 h; brighter cellular fluorescence vs FAM-HDOdna after 2 h
Output Units
Output Notes	Fluorescence microscopy: after 2 h, punctate fluorescence co-localized with LysoTracker Red, indicating most internalized decoys remained within endosomes/lysosomes (endosomal entrapment).
Toxicity Notes	Cell proliferation assessed by WST-8 viability assay (no explicit toxicity statement reported).
Curation Notes