Hydrophobic CPP/HDO conjugates: a new frontier in oligonucleotide-warheaded PROTAC delivery (2024)
Sequence: LGAQSNF
decoy oligonucleotide PROTAC (DNA)
| Experiment Id | EXP001042 |
|---|---|
| Paper | Hydrophobic CPP/HDO conjugates: a new frontier in oligonucleotide-warheaded PROTAC delivery |
| Peptide | P4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10 µM P4 (co-treatment; no transfection) |
| Rna Concentration | 10 µM LCL-ER(dec) (co-treatment; no transfection) |
| Mixing Ratio | co-treatment mixture (LCL-ER(dec) + P4; ratio not specified; same nominal concentration 10 µM each) |
| Formulation Format | mixture / co-treatment (noncovalent; no transfection reagent) |
| Formulation Components | LCL-ER(dec) + free P4 peptide |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 (human breast cancer cells; ERα-positive) |
| Animal Model | |
| Administration Route | |
| Output Type | ERα degradation |
| Output Value | ERα degradation observed at 10 µM in LCL-ER(dec)+P4 co-treatment after 24 h (no transfection) |
| Output Units | |
| Output Notes | Paper notes ERα degradation in co-treatment at high concentration; P4 alone did not induce ERα degradation. |
| Toxicity Notes | |
| Curation Notes |