Hydrophobic CPP/HDO conjugates: a new frontier in oligonucleotide-warheaded PROTAC delivery (2024)
Sequence: LGAQSNF
decoy oligonucleotide PROTAC (DNA)
| Experiment Id | EXP001043 |
|---|---|
| Paper | Hydrophobic CPP/HDO conjugates: a new frontier in oligonucleotide-warheaded PROTAC delivery |
| Peptide | P4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | tested (Fig. S6†); little ERα degrading activity reported |
| Rna Concentration | tested (Fig. S6†); little ERα degrading activity reported |
| Mixing Ratio | N/A (covalent conjugate) |
| Formulation Format | covalent conjugate (LCL-ER(dec)-P4) |
| Formulation Components | LCL-ER(dec)-P4 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 (human breast cancer cells; ERα-positive) |
| Animal Model | |
| Administration Route | |
| Output Type | ERα degradation |
| Output Value | Little ERα degrading activity (Fig. S6†) |
| Output Units | |
| Output Notes | Direct P4 conjugation to LCL-ER(dec) reduced ERα degradation compared with CPP/HDO-PROTAC or LCL-ER(dec)+P4 co-treatment. |
| Toxicity Notes | |
| Curation Notes |